Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics.
Identifieur interne : 000B29 ( Main/Exploration ); précédent : 000B28; suivant : 000B30Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics.
Auteurs : Gorugantu Sribala [Belgique] ; Hilal Ezgi Toraman [Belgique] ; Steffen Symoens [Belgique] ; Annabelle Déjardin [France] ; Gilles Pilate [France] ; Wout Boerjan [Belgique] ; Frederik Ronsse [Belgique] ; Kevin M. Van Geem [Belgique] ; Guy B. Marin [Belgique]Source :
- Computational and structural biotechnology journal [ 2001-0370 ] ; 2019.
Abstract
Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (CAD), CAFFEIC ACID O-METHYLTRANSFERASE (COMT) and CAFFEOYL-CoA O-METHYLTRANSFERASE (CCoAOMT), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by COMT, CAD and CCoAOMT enzyme downregulation. COMT and CAD downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The sCAD line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while CCoAOMT downregulation affected the least (7-11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the CAD transgenic lines and its wild type. On the other hand, COMT downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.
DOI: 10.1016/j.csbj.2019.04.007
PubMed: 31080566
PubMed Central: PMC6502739
Affiliations:
- Belgique, France
- Centre-Val de Loire, Province de Flandre-Orientale, Région Centre, Région flamande
- Gand, Orléans
- Université de Gand
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sort="Ronsse, Frederik" uniqKey="Ronsse F" first="Frederik" last="Ronsse">Frederik Ronsse</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Geem, Kevin M" sort="Van Geem, Kevin M" uniqKey="Van Geem K" first="Kevin M" last="Van Geem">Kevin M. 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nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Toraman, Hilal Ezgi" sort="Toraman, Hilal Ezgi" uniqKey="Toraman H" first="Hilal Ezgi" last="Toraman">Hilal Ezgi Toraman</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Symoens, Steffen" sort="Symoens, Steffen" uniqKey="Symoens S" first="Steffen" last="Symoens">Steffen Symoens</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name><affiliation wicri:level="3"><nlm:affiliation>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Orléans</settlement></placeName></affiliation></author><author><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent</wicri:regionArea><wicri:noRegion>9052 Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Ronsse, Frederik" sort="Ronsse, Frederik" uniqKey="Ronsse F" first="Frederik" last="Ronsse">Frederik Ronsse</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent</wicri:regionArea><placeName><region type="province" nuts="2">Province de Flandre-Orientale</region><settlement type="city">Gand</settlement></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Geem, Kevin M" sort="Van Geem, Kevin M" uniqKey="Van Geem K" first="Kevin M" last="Van Geem">Kevin M. Van Geem</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author><author><name sortKey="Marin, Guy B" sort="Marin, Guy B" uniqKey="Marin G" first="Guy B" last="Marin">Guy B. Marin</name><affiliation wicri:level="4"><nlm:affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent</wicri:regionArea><orgName type="university">Université de Gand</orgName><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName></affiliation></author></analytic><series><title level="j">Computational and structural biotechnology journal</title><idno type="ISSN">2001-0370</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (<i>CAD</i>), CAFFEIC ACID O-METHYLTRANSFERASE (<i>COMT</i>) and CAFFEOYL-CoA O-METHYLTRANSFERASE (<i>CCoAOMT</i>), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by <i>COMT</i>, <i>CAD</i> and <i>CCoAOMT</i> enzyme downregulation. <i>COMT</i> and <i>CAD</i> downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The s<i>CAD</i> line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while <i>CCoAOMT</i> downregulation affected the least (7-11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the <i>CAD</i> transgenic lines and its wild type. On the other hand, <i>COMT</i> downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31080566</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2001-0370</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Computational and structural biotechnology journal</Title><ISOAbbreviation>Comput Struct Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Analytical Py-GC/MS of Genetically Modified Poplar for the Increased Production of Bio-aromatics.</ArticleTitle><Pagination><MedlinePgn>599-610</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.csbj.2019.04.007</ELocationID><Abstract><AbstractText>Genetic engineering is a powerful tool to steer bio-oil composition towards the production of speciality chemicals such as guaiacols, syringols, phenols, and vanillin through well-defined biomass feedstocks. Our previous work demonstrated the effects of lignin biosynthesis gene modification on the pyrolysis vapour compositions obtained from wood derived from greenhouse-grown poplars. In this study, field-grown poplars downregulated in the genes encoding CINNAMYL ALCOHOL DEHYDROGENASE (<i>CAD</i>), CAFFEIC ACID O-METHYLTRANSFERASE (<i>COMT</i>) and CAFFEOYL-CoA O-METHYLTRANSFERASE (<i>CCoAOMT</i>), and their corresponding wild type were pyrolysed in a Py-GC/MS. This work aims at capturing the effects of downregulation of the three enzymes on bio-oil composition using principal component analysis (PCA). 3,5-methoxytoluene, vanillin, coniferyl alcohol, 4-vinyl guaiacol, syringol, syringaldehyde, and guaiacol are the determining factors in the PCA analysis that are the substantially affected by <i>COMT</i>, <i>CAD</i> and <i>CCoAOMT</i> enzyme downregulation. <i>COMT</i> and <i>CAD</i> downregulated transgenic lines proved to be statistically different from the wild type because of a substantial difference in S and G lignin units. The s<i>CAD</i> line lead to a significant drop (nearly 51%) in S-lignin derived compounds, while <i>CCoAOMT</i> downregulation affected the least (7-11%). Further, removal of extractives via pretreatment enhanced the statistical differences among the <i>CAD</i> transgenic lines and its wild type. On the other hand, <i>COMT</i> downregulation caused 2-fold reduction in S-derived compounds compared to G-derived compounds. This study manifests the applicability of PCA analysis in tracking the biological changes in biomass (poplar in this case) and their effects on pyrolysis-oil compositions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>SriBala</LastName><ForeName>Gorugantu</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Toraman</LastName><ForeName>Hilal Ezgi</ForeName><Initials>HE</Initials><AffiliationInfo><Affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Symoens</LastName><ForeName>Steffen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Déjardin</LastName><ForeName>Annabelle</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pilate</LastName><ForeName>Gilles</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institut National de la Recherche Agronomique (INRA), Unité de Recherche 0588, Amélioration, Génétique et Physiologie Forestières, 45075 Orléans, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>Wout</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ronsse</LastName><ForeName>Frederik</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Geem</LastName><ForeName>Kevin M</ForeName><Initials>KM</Initials><AffiliationInfo><Affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marin</LastName><ForeName>Guy B</ForeName><Initials>GB</Initials><AffiliationInfo><Affiliation>Ghent University, Laboratory for Chemical Technology, Technologiepark 125, 9052 Ghent, Belgium.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>04</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Comput Struct Biotechnol J</MedlineTA><NlmUniqueID>101585369</NlmUniqueID><ISSNLinking>2001-0370</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Analytical fast pyrolysis</Keyword><Keyword MajorTopicYN="N">C, Holocellulose</Keyword><Keyword MajorTopicYN="N">CAD, CINNAMYL ALCOHOL DEHYDROGENASE</Keyword><Keyword MajorTopicYN="N">CCoAOMT, CAFFEOYL-CoA O-METHYLTRANSFERASE</Keyword><Keyword MajorTopicYN="N">COMT, CAFFEIC ACID O-METHYLTRANSFERASE</Keyword><Keyword MajorTopicYN="N">G, Guaiacyl units</Keyword><Keyword MajorTopicYN="N">GC, Gas chromatography</Keyword><Keyword MajorTopicYN="N">Genetically modified poplar</Keyword><Keyword MajorTopicYN="N">H, p-hydroxyphenyl units</Keyword><Keyword MajorTopicYN="N">L, Lignin-derived aromatic compounds</Keyword><Keyword MajorTopicYN="N">L-G, Guaiacyl lignin-derived compounds</Keyword><Keyword MajorTopicYN="N">L-H, p-Hydroxyphenyl lignin-derived compounds</Keyword><Keyword MajorTopicYN="N">L-S, Syringyl lignin-derived compounds</Keyword><Keyword MajorTopicYN="N">Lignin</Keyword><Keyword MajorTopicYN="N">MD, Mahalanobis distance</Keyword><Keyword MajorTopicYN="N">MS, Mass spectroscopy</Keyword><Keyword MajorTopicYN="N">PC, Principal component</Keyword><Keyword MajorTopicYN="N">Phenolic compounds</Keyword><Keyword MajorTopicYN="N">Principal component analysis</Keyword><Keyword MajorTopicYN="N">Py, Micropyrolysis or micropyrolyzer</Keyword><Keyword MajorTopicYN="N">S, Syringyl units</Keyword><Keyword MajorTopicYN="N">as, Antisense line</Keyword><Keyword MajorTopicYN="N">s, Sense line</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>12</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>14</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31080566</ArticleId><ArticleId IdType="doi">10.1016/j.csbj.2019.04.007</ArticleId><ArticleId IdType="pii">S2001-0370(18)30351-9</ArticleId><ArticleId 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sort="Sribala, Gorugantu" uniqKey="Sribala G" first="Gorugantu" last="Sribala">Gorugantu Sribala</name></region><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name><name sortKey="Marin, Guy B" sort="Marin, Guy B" uniqKey="Marin G" first="Guy B" last="Marin">Guy B. Marin</name><name sortKey="Ronsse, Frederik" sort="Ronsse, Frederik" uniqKey="Ronsse F" first="Frederik" last="Ronsse">Frederik Ronsse</name><name sortKey="Symoens, Steffen" sort="Symoens, Steffen" uniqKey="Symoens S" first="Steffen" last="Symoens">Steffen Symoens</name><name sortKey="Toraman, Hilal Ezgi" sort="Toraman, Hilal Ezgi" uniqKey="Toraman H" first="Hilal Ezgi" last="Toraman">Hilal Ezgi Toraman</name><name sortKey="Van Geem, Kevin M" sort="Van Geem, Kevin M" uniqKey="Van Geem K" first="Kevin M" last="Van Geem">Kevin M. Van Geem</name></country><country name="France"><region name="Centre-Val de Loire"><name sortKey="Dejardin, Annabelle" sort="Dejardin, Annabelle" uniqKey="Dejardin A" first="Annabelle" last="Déjardin">Annabelle Déjardin</name></region><name sortKey="Pilate, Gilles" sort="Pilate, Gilles" uniqKey="Pilate G" first="Gilles" last="Pilate">Gilles Pilate</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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